monoclonal anti-hctr1 antibody  (Novus Biologicals)

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Rapid diagnosis of cisplatin-sensitive and resistant cervical squamous cell carcinomas by reverse transcription loop-mediated isothermal amplification

doi:

Figure Lengend Snippet: Primer design for the detection of hCTR1 mRNA by RT-LAMP

Article Snippet: One half was frozen within 30 min after surgery in liquid nitrogen and stored at -80°C until molecular analysis, while the other half was fixed with 10% buffered formalin and embedded in paraffin for routine histopathological examination with H&E staining and IHC examination with monoclonal anti-hCTR1 antibody (Novus, Littleton, USA).

Techniques: Sequencing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Rapid diagnosis of cisplatin-sensitive and resistant cervical squamous cell carcinomas by reverse transcription loop-mediated isothermal amplification

doi:

Figure Lengend Snippet: Sensitivity of LAMP reaction to detect hCTR1. A. The turbidity curve of LAMP technique using a known number of hCTR1 cell copies after 60 min amplification. B. The result of LAMP reaction after 45 min amplification measured by turbidimeter. Lane 1 to 7 was loaded with serially diluted hCTR1 cell copies from one million to one. Lane 8 was loaded as ddH2O as blank control.

Article Snippet: One half was frozen within 30 min after surgery in liquid nitrogen and stored at -80°C until molecular analysis, while the other half was fixed with 10% buffered formalin and embedded in paraffin for routine histopathological examination with H&E staining and IHC examination with monoclonal anti-hCTR1 antibody (Novus, Littleton, USA).

Techniques: Amplification, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Rapid diagnosis of cisplatin-sensitive and resistant cervical squamous cell carcinomas by reverse transcription loop-mediated isothermal amplification

doi:

Figure Lengend Snippet: Representative hCTR1 over-expressed (A) cervical squamous cell carcinoma and low-expressed carcinoma (B) cases analyzed by immunohistochemical staining and RT-LAMP. In RT-LAMP reaction, carcinoma RNA was loaded into lane 1 to 4 as duplicates, while adjacent normal tissue RNA was loaded into lane 5 to 8 as duplicates. *, P<0.05; **, P>0.05.

Article Snippet: One half was frozen within 30 min after surgery in liquid nitrogen and stored at -80°C until molecular analysis, while the other half was fixed with 10% buffered formalin and embedded in paraffin for routine histopathological examination with H&E staining and IHC examination with monoclonal anti-hCTR1 antibody (Novus, Littleton, USA).

Techniques: Immunohistochemical staining, Staining